They were charged with security breaches and failure to protect her, prosecutors said.
Five alleged Taliban militants have also been charged with criminal conspiracy over Bhutto’s death.
She died in a gun and bomb attack while campaigning for election Pervez Musharraf, president of Pakistan at the time, has also been implicated in Ms Bhutto’s murder.
The anti-terrorism court issued an arrest warrant for him in February over what it said was his failure to provide her with adequate security.
He was declared a fugitive after failing to appear at the court in February.
Mr Musharraf denies the allegation.
In.
The five suspected Taliban members have been in custody for nearly four years.
They are accused of bringing a suicide bomber from Pakistan’s tribal areas to Rawalpindi to carry out the attack.
Three other suspects, including the head of the Pakistani Taliban Baitullah Mehsud, have been killed in a US drone strike in 2009.
After Ms Bhutto’s death, her Pakistan People’s Party won general elections and her widower, Asif Ali Zardari, became president after Mr Musharraf was forced to step down.
Ms Bhutto was Pakistan’s prime minister from from 1988 to 1990 and from 1993 to 1996